110V轉(zhuǎn)正負(fù)15V電源模塊,DC-DC模塊電源,150W電源
北京誠遠(yuǎn)信達(dá)電子科技有限公司
專業(yè)從事AC-DC,DC-DC開關(guān)電源及模塊電源的研究開發(fā)及制造,是國內(nèi)較早從事電源研發(fā)生產(chǎn)的廠家之一,公司為中國電源協(xié)會(huì)成員單位。公司產(chǎn)品廣泛應(yīng)用于工控、通訊、電力、醫(yī)療、科研、軍工、安防、LED、儀器儀表、航天航空、船舶機(jī)械等領(lǐng)域。
聯(lián)系人:宋愛民 先生
電話:18612703179
QQ : 953617934
流式細(xì)胞儀測定。(4×105/孔,6孔板,2mL/孔) 若第二天流式測量,可加5—10mL70%乙醇,在4℃固定8h以上。
Caco-2細(xì)胞 人結(jié)直腸腺癌細(xì)胞 http://www.afzhan.com/st133337/Product_3325168.html
CaSki細(xì)胞 人宮頸癌細(xì)胞 CaSki細(xì)胞傳代 http://www.afzhan.com/st133337/Product_4632941.html
制備細(xì)胞凋亡單染管: 活細(xì)胞
一半活細(xì)胞+一半死細(xì)胞(高溫滅活-水浴90℃)+FITC(染色活Cell)
一半活細(xì)胞+一半死細(xì)胞+PI(PI-碘化丙啶染色核酸,但不能穿透細(xì)胞膜即染色死Cell)
Caco-1細(xì)胞人結(jié)直腸細(xì)胞/Caco-2細(xì)胞傳代 http://www.afzhan.com/st133337/Product_4632937.html
Caco-2細(xì)胞直腸腺癌細(xì)胞 Caco-2細(xì)胞價(jià)格 http://www.afzhan.com/st133337/Product_4592202.html
一半活細(xì)胞+一半死細(xì)胞+FITC(堿性環(huán)境與抗體蛋白反應(yīng),主要是與賴氨酸的氨基與熒光素的硫碳胺鍵結(jié)合,形成FITC-蛋白質(zhì)結(jié)合)+PI PBS的配制:(1000mL) NaCl 8.0 g 、KCl 0.2 g、 KH2PO4 0.2 g 、Na2HPO4.12H2O 2.9 g 加超純水至1000mL高壓蒸汽滅菌,4℃儲(chǔ)存,備用。 細(xì)胞器具清洗: 瓶、管等+潔消精(過夜12 h),自來水沖洗25遍,蒸餾水
BS-C-1細(xì)胞洲綠猴腎細(xì)胞 BS-C-1細(xì)胞價(jià)格 http://www.afzhan.com/st133337/Product_4592195.html
BS-C-1細(xì)胞 非洲綠猴腎細(xì)胞 http://www.afzhan.com/st133337/Product_3324740.html
沖1-2遍,蒸餾水泡過夜12 h,烘干,高壓蒸汽滅菌(約2 h),再烘干,至少2-3天,方可用。
MTT溶液的配制:MTT濃度為5 mg/mL,溶于PBS或無酚紅的培養(yǎng)基中,用0.22 um濾膜過濾以除去溶液里的細(xì)菌,放4℃避光保存即可。
人黑色素瘤細(xì)胞 A875細(xì)胞 / A875細(xì)胞價(jià)格 http://www.afzhan.com/st133337/Product_4632928.html
A875細(xì)胞 人黑色素瘤細(xì)胞 A875細(xì)胞培養(yǎng) http://www.afzhan.com/st133337/Product_4592191.html
A875細(xì)胞 人黑色素瘤細(xì)胞 http://www.afzhan.com/st133337/Product_3324708.html
注:
在酶標(biāo)儀檢測結(jié)果時(shí),為保證實(shí)驗(yàn)結(jié)果的線性,MTT吸光度最好在0-0.7范圍;
人乳腺導(dǎo)管癌細(xì)胞ZR751細(xì)胞/ZR751細(xì)胞價(jià)格 http://www.afzhan.com/st133337/Product_4592182.html
ZR751細(xì)胞 人乳腺導(dǎo)管癌細(xì)胞 http://www.afzhan.com/st133337/Product_3324675.html
人乳腺導(dǎo)管癌細(xì)胞 ZR-75-1細(xì)胞 http://www.afzhan.com/st133337/Product_4394341.html
MTT一般現(xiàn)用現(xiàn)配,過濾后4℃,避光保存2周有效,或保存在-20℃長期保存;避免反復(fù)凍融,最好小劑量分裝,用避光袋、錫箔紙包裹;一般把MTT粉分裝在EP管,再現(xiàn)用現(xiàn)配,當(dāng)MTT變?yōu)榛揖G色時(shí)絕不能再用; MTT致癌,用時(shí)最好帶透明薄膜手套。
電氣連接: | 導(dǎo)線、焊盤、導(dǎo)線帶、導(dǎo)柱 |
類型: | 表壓,絕壓 316SS不銹鋼隔離膜片 |
特點(diǎn): | 壓力1PSI,O型密封圈或螺紋連接 |
供電電源: | 1.5mA |
輸出: | 0~100mV |
精確度: | ±0.25%非線性 |
工作溫度范圍: | -40℃~125℃ (補(bǔ)償:-20~85℃) |
量程: | 0~1,5(psi) |
典型應(yīng)用: | 壓力變送器,醫(yī)療儀器,試驗(yàn)設(shè)備,真空裝置 |
【先行者通用型天平 CP系列 0.001g的參數(shù)說明】 | |||||||||||||||||||||||||||||||||||||||
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大鼠生長分化因子15(GDF15)elisa試劑盒
本試劑盒只能用于科學(xué)研究,不得用于醫(yī)學(xué)診斷
檢測原理
試劑盒采用雙抗體一步夾心法酶聯(lián)免疫吸附試驗(yàn)(ELISA)。往預(yù)先包被大鼠生長分化因子15(GDF15)捕獲抗體的包被微孔中,依次加入標(biāo)本、標(biāo)準(zhǔn)品、HRP標(biāo)記的檢測抗體,經(jīng)過溫育并洗滌。用底物TMB顯色,TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的大鼠生長分化因子15(GDF15)呈正相關(guān)。用酶標(biāo)儀在450nm 波長下測定吸光度(OD 值),計(jì)算樣品濃度。
樣品收集、處理及保存方法
1. 血清:使用不含熱原和內(nèi)毒素的試管,操作過程中避免任何細(xì)胞刺激,收集血液后,3000轉(zhuǎn)離心10分鐘將血清和紅細(xì)胞迅速小心地分離。
2. 血漿:EDTA、檸檬酸鹽或肝素抗凝。3000轉(zhuǎn)離心30分鐘取上清。
3. 細(xì)胞上清液:3000轉(zhuǎn)離心10分鐘去除顆粒和聚合物。
4. 組織勻漿:將組織加入適量生理鹽水搗碎。3000轉(zhuǎn)離心10分鐘取上清。
5. 保存:如果樣本收集后不及時(shí)檢測,請按一次用量分裝,凍存于-20℃,避免反復(fù)凍融,在室溫下解凍并確保樣品均勻地充分解凍。
自備物品
1. 酶標(biāo)儀(450nm)
2. 高精度加樣器及槍頭:0.5-10uL、2-20uL、20-200uL、200-1000uL
3. 37℃恒溫箱
操作注意事項(xiàng)
1. 試劑盒保存在2-8℃,使用前室溫平衡20分鐘。從冰箱取出的濃縮洗滌液會(huì)有結(jié)晶,這屬于正,F(xiàn)象,水浴加熱使結(jié)晶溶解后再使用。
2. 實(shí)驗(yàn)中不用的板條應(yīng)立即放回自封袋中,密封(低溫干燥)保存。
3. 預(yù)處理后的樣本無需稀釋,直接取10μL加樣即可。
4. 嚴(yán)格按照說明書中標(biāo)明的時(shí)間、加液量及順序進(jìn)行溫育操作。
5. 所有液體組分使用前充分搖勻。
試劑盒組成
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微孔酶標(biāo)板 | | | |
標(biāo)準(zhǔn)品(1600 pg/ml) | | | |
標(biāo)準(zhǔn)品稀釋液 | | | |
樣本稀釋液 | | | |
檢測抗體-HRP | | | |
20×洗滌緩沖液 | | | |
底物A | | | |
底物B | | | |
終止液 | | | |
封板膜 | | | |
說明書 | | | |
自封袋 | | | |
注:標(biāo)準(zhǔn)品用標(biāo)準(zhǔn)品稀釋液依次稀釋為:1600、800、400、200、100、50 pg/mL.
試劑的準(zhǔn)備
20×洗滌緩沖液的稀釋:蒸餾水按1:20稀釋,即1份的20×洗滌緩沖液加19份的蒸餾水。
洗板方法
1. 手工洗板:甩盡孔內(nèi)液體,每孔加滿洗滌液,靜置1min后甩盡孔內(nèi)液體,在吸水紙上拍干,如此洗板5次。
2. 自動(dòng)洗板機(jī):每孔注入洗液350μL,浸泡1min,洗板5次。
操作步驟
1. 從室溫平衡20min后的鋁箔袋中取出所需板條,剩余板條用自封袋密封放回4℃。
2. 設(shè)置標(biāo)準(zhǔn)品孔、樣本孔和空白孔,空白孔什么都不加;標(biāo)準(zhǔn)品孔各加不同濃度的標(biāo)準(zhǔn)品50μL;
3. 待測樣本孔先加待測樣本10μL,再加樣本稀釋液40μL;
4. 隨后標(biāo)準(zhǔn)品孔和樣本孔中(空白孔不加)加入辣根過氧化物酶(HRP)標(biāo)記的檢測抗體100μL,,用封板膜封住反應(yīng)孔,37℃水浴鍋或恒溫箱溫育60min。
5. 棄去液體,吸水紙上拍干,每孔加滿洗滌液,靜置1min,甩去洗滌液,吸水紙上拍干,如此重復(fù)洗板5次(也可用洗板機(jī)洗板)。
6. 所有孔加入底物A、B各50μL,37℃避光孵育15min。
7. 所有孔加入終止液50μL,15min內(nèi),在450nm波長處測定各孔的OD值。
結(jié)果判斷
繪制標(biāo)準(zhǔn)曲線:在Excel工作表中,以標(biāo)準(zhǔn)品濃度作橫坐標(biāo),對應(yīng)OD值作縱坐標(biāo),繪制出標(biāo)準(zhǔn)品線性回歸曲線,按曲線方程計(jì)算各樣本濃度值。
試劑盒性能
1. 性:標(biāo)準(zhǔn)品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值,大于等于0.9900。
2. 靈敏度:檢測濃度小于10 pg/mL。
3. 特異性:不與其它可溶性結(jié)構(gòu)類似物交叉反應(yīng)。
4. 重復(fù)性:板內(nèi)、板間變異系數(shù)均小于15%。
5. 貯藏:2-8℃,避光防潮保存。
6. 期:6個(gè)月
免責(zé)聲明
1. 試劑盒僅供研究使用,不得用于臨床實(shí)驗(yàn)或人體實(shí)驗(yàn),否則所產(chǎn)生的一切后果,由實(shí)驗(yàn)者承擔(dān),本公司概不負(fù)責(zé)。
2. 嚴(yán)格按照說明書操作,實(shí)驗(yàn)者違反說明書操作,后果由實(shí)驗(yàn)者承擔(dān)。
FOR RESEARCH USE ONLY.
NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Intended use
This GDF15 ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of GDF15 in the sample, this GDF15 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus GDF15 concentration. The concentration of GDF15 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Sample collection and storages
Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles
Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.
Materials required but not supplied
1. Standard microplate reader(450nm)
2. Precision pipettes and Disposable pipette tips.
3. 37 ℃ incubator
Precautions
1. Do not substitute reagents from one kit to another. Standard, conjugate and microplates are matched for optimal performance. Use only the reagents supplied by manufacturer.
2. Do not remove microplate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.
3. Mix all reagents before using.
Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C)
Materials supplied
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Note: Standard diluent with Standard diluent concentration was followed by:
1600、800、400、200、100、50 pg/mL.
Reagent preparation
Assay procedure
1. Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.
2. Add standard: Set Standard wells, testing sample wells. Blank well doesn’t add anyting. Add standard 50μl to standard well.
3. Add Sample: Blank well doesn’t add anyting. Add testing sample 10μl Then add sample diluent 40μl to testing sample well.
4. Blank well doesn’t add anyting. Add 100μl of HRP-conjugate reagent to standard wells and sample wells, cover with an adhesive strip and incubate for 60 minutes at 37°C.
5. Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.
7. Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not
appear uniform, gently tap the plate to ensure thorough mixing.
8. Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.
Calculation of results
1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.
2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.
3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.
4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
5. The sensitivity by this assay is 10 pg/mL.
6. Standard curve
Storage: 2-8℃.
validity: six months.
■ 使用條件 |
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、泵的使用環(huán)境溫度≤40℃,濕度≤95%;海拔高度≤1000米。A B、介質(zhì)溫度不超過60℃,介質(zhì)重度為1~1.3kg/dm3。 C、鑄鐵材質(zhì)的使用范圍這PH5~9。 D、不銹鋼材質(zhì)可使用一般腐蝕性介質(zhì)。 E、使用環(huán)境海拔高度不超過1000米,超過以上條件時(shí),應(yīng)在訂貨時(shí)提出,以便為您提供更可靠的產(chǎn)品。 注:用戶如有特殊的溫度,介質(zhì)等要求,請?jiān)谟嗀洉r(shí)注明輸送介質(zhì)的詳細(xì)情況,以便本單位提供更為可靠之產(chǎn)品。 |
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■ 產(chǎn)品特點(diǎn) |
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1、采用雙葉片葉輪結(jié)構(gòu),大大提高了污物的通過能力 2、機(jī)械密封采用新型磨檫副,并長期處入油室內(nèi)運(yùn)行; 3、整體結(jié)構(gòu)緊湊、體積小、燥聲小、節(jié)能效果顯著,檢修方便,方便用戶更換; 4、自動(dòng)控制柜可以根據(jù)所需液化變化,自動(dòng)控制泵的超動(dòng)與停止,不需專人看管,使用極為方便; 5、可根據(jù)用戶需要配備安裝方式,它給安裝、維修到來極大的方便,人可不必此為而進(jìn)入污水坑; 6、能夠在設(shè)計(jì)范圍內(nèi)使用,而保證電機(jī)不會(huì)過載; 7、該泵配上戶外性電機(jī),則無須建泵房,可直接安裝與戶外使用節(jié)省開支; |
此類激光二極管模塊是帶單模態(tài)光纖尾纖的高穩(wěn)定度激光二極管模塊,這些模塊是用于高速光通信系統(tǒng)和測量儀器最理想的光源。 特性: 每小時(shí)功率穩(wěn)定性- <0.02dB 模塊輸出- <2 minutes LD工作哦電壓- <1.5 V 內(nèi)置InGaAs監(jiān)控光電二極管 監(jiān)控光電二極管電流 >0.2mA 跟蹤誤差- <± 0.8 dB 極值標(biāo)準(zhǔn) LD反向電壓 2v PD反向電壓 20v 安全工作溫度 -20~+50°c 儲(chǔ)存溫度 -30~+60°c 參數(shù) 電/光學(xué) 性能 (SM,T=25°c) 型號 波長nm 閾值電流mA 工作電流mA 斜率效率 上升和下降時(shí)間 輸出功率 mW/mA nom max LDI H-FP-1310-30P 1310 ± 20 6 <120 >0.24 0.3 0.5 30 LDI H-DFB-1310-30P 1310 ± 20 6 <120 >0.24 0.1 0.2 30 LDI H-FP-1550-15P 1550 ± 20 8 <120 >0.16 0.3 0.5 15 LDI H-DFB-1550-15P 1550 ± 20 8 <120 >0.16 0.08 0.12 15 需要更詳細(xì)的咨詢,請與邁可諾中國營銷服務(wù)中心聯(lián)系 Tel:021-51078515(上海) 010-52914751(北京) 027-50486618(武漢) Toll Free:400-8800-298 Email:sales@cro.com.cn |
水處理專用流量計(jì)
流量選擇:
測液體:400-4000L/h 630-6300L/h 1-10M3/h
測氣體: 12-120m3/h 20-200M3/h
口徑:15mm
材質(zhì) :外殼碳鋼 接頭304不銹鋼材質(zhì) 螺紋連接 (可訂做衛(wèi)生卡箍連接)
G10-15玻璃轉(zhuǎn)子流量計(jì) 鋁合金材質(zhì)螺紋連接 外觀精美首選常州良邦?
VA10型玻璃轉(zhuǎn)子流量計(jì)最大工作壓力和最高工 作溫度均高于原有相同通徑的LZB/LZJ系列玻璃轉(zhuǎn)子流量計(jì)產(chǎn)品;分普通型(測量一般無腐蝕流體)和耐腐型(測量的腐蝕性流體)兩種。產(chǎn)品體積小、結(jié)構(gòu)輕巧,外形美觀,維修方便、安全可靠,安裝簡單。F10、20、30型法蘭可轉(zhuǎn)動(dòng),與現(xiàn)場管道法蘭孔吻合。 VA/FA 型玻璃轉(zhuǎn)子流量計(jì)可替代引進(jìn)成套設(shè)備上的玻轉(zhuǎn)儀表,廣泛應(yīng)用于化工、輕工、制藥、食品、造紙、環(huán)保和計(jì)量測試、科研等部門,測量單相非脈動(dòng)流體(液體或氣體)的流量。
G10-15玻璃轉(zhuǎn)子流量計(jì) 鋁合金材質(zhì)螺紋連接 外觀精美首選常州良邦